Question 1

If the decolorizer is not left on long enough in the Gram-staining procedure,gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Accepted Answer

A)  purple; purple
B)  purple; colorless
C)  purple; pink
D)  pink; pinkE) All of the above
F) C) and D)

Question 2

Scanning electron microscopy is most often used to reveal

Accepted Answer

A)  surface structures.
B)  internal structures.
C)  both surface and internal structures simultaneously.
D)  either surface or internal structures,but not simultaneously.E) None of the above
F) A) and B)

Question 3

After the secondary stain has been added,gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

Accepted Answer

A)  purple; purple
B)  purple; colorless
C)  purple; pink
D)  pink; pinkE) B) and C)
F) A) and B) C

Question 4

As the magnification of a series of objective lenses increases,the working distance

Accepted Answer

A)  increases.
B)  decreases.
C)  stays the same.
D)  cannot be predicted.E) A) and C)
F) B) and D)

Question 5

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized.

Accepted Answer

The answer of The useful magnification of a light microscope...

Question 6

Acid-fast organisms such as Mycobacterium tuberculosis contain __________ constructed from mycolic acids in their cell walls.

Accepted Answer

A)  proteins
B)  carbohydrates
C)  lipids
D)  peptidoglycanE) A) and B)
F) C) and D)

Question 7

The Gram-staining procedure is an example of:

Accepted Answer

A)  simple staining.
B)  negative staining.
C)  differential staining.
D)  fluorescent staining.E) A) and B)
F) None of the above

Question 8

A 45 ×	imes×  objective and a 10 ×	imes×  ocular produce a total magnification of

Accepted Answer

A)  900 ×	imes× .
B)  55 ×	imes× .
C)  450 ×	imes× .
D)  145 ×	imes× . E) B) and D)
F) B) and C)

Question 9

__________ breaks frozen specimens along lines of greatest weakness,often down the middle of lipid bilayer membranes so that they may be observed by transmission electron microscopy.

Accepted Answer

The answer of __________ breaks frozen specimens along lines of...

Question 10

A substage condenser is used to focus light onto the specimen,which increases the resolution of a light microscope.

Accepted Answer

A) True 
 B)False

Question 11

The procedure in which a single stain is used to visualize microorganisms is called __________ staining.

Accepted Answer

The answer of The procedure in which a single stain...

Question 12

Because transmission electron microscopy uses electrons rather than light,it is not necessary to stain biological specimens before observing them.

Accepted Answer

A) True 
 B)False

Question 13

Scanning electron microscopes bombard specimens with a stream of electrons; however,the specimen image is produce by electrons that are derived from atoms of the specimen itself rather than by the electrons used to bombard the specimen.

Accepted Answer

A) True 
 B)False  True

Question 14

In order to stain flagella so that they may be readily observed by light microscopy,it is usually necessary to increase their thickness.

Accepted Answer

A) True 
 B)False

Question 15

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Accepted Answer

A)  optical density
B)  refractive index
C)  optical density and refractive index
D)  neither optical density nor refractive indexE) C) and D)
F) A) and D) B

Question 16

Light rays are refracted (bent)when they cross the interface between materials with different refractive indices.

Accepted Answer

A) True 
 B)False

Question 17

In the Gram-staining procedure,the counterstain is

Accepted Answer

A)  iodine.
B)  safranin.
C)  crystal violet.
D)  alcohol.E) All of the above
F) B) and C)

Question 18

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n) __________ microscope.

Accepted Answer

A)  phase-contrast
B)  electron
C)  fluorescence
D)  densitometricE) A) and B)
F) A) and C)

Question 19

An electron microscope uses __________ lenses to focus beams of electrons onto a specimen.

Accepted Answer

The answer of An electron microscope uses __________ lenses to...

Question 20

The designer of the first transmission electron microscope,_________________,was awarded the 1986 Nobel Prize in physics.

Accepted Answer

The answer of The designer of the first transmission electron...

Exam 2: Microscopy

If the decolorizer is not left on long enough in the Gram-staining procedure,gram-positive organisms will be stained __________ and gram-negative organisms will be stained __________.

Correct AnswerverifiedShow Answer

Scanning electron microscopy is most often used to reveal

Correct AnswerverifiedShow Answer

After the secondary stain has been added,gram-positive organisms are stained __________ and gram-negative organisms are stained __________.

Correct AnswerverifiedC

As the magnification of a series of objective lenses increases,the working distance

Correct AnswerverifiedShow Answer

The useful magnification of a light microscope is limited by the ___________ of the light source being utilized.

Correct AnswerverifiedShow Answer

Acid-fast organisms such as Mycobacterium tuberculosis contain __________ constructed from mycolic acids in their cell walls.

Correct AnswerverifiedShow Answer

The Gram-staining procedure is an example of:

Correct AnswerverifiedShow Answer

A 45 ×\times× objective and a 10 ×\times× ocular produce a total magnification of

Correct AnswerverifiedShow Answer

__________ breaks frozen specimens along lines of greatest weakness,often down the middle of lipid bilayer membranes so that they may be observed by transmission electron microscopy.

Correct AnswerverifiedShow Answer

A substage condenser is used to focus light onto the specimen,which increases the resolution of a light microscope.

Correct AnswerverifiedShow Answer

The procedure in which a single stain is used to visualize microorganisms is called __________ staining.

Correct AnswerverifiedShow Answer

Because transmission electron microscopy uses electrons rather than light,it is not necessary to stain biological specimens before observing them.

Correct AnswerverifiedShow Answer

Scanning electron microscopes bombard specimens with a stream of electrons; however,the specimen image is produce by electrons that are derived from atoms of the specimen itself rather than by the electrons used to bombard the specimen.

Correct AnswerverifiedTrue

In order to stain flagella so that they may be readily observed by light microscopy,it is usually necessary to increase their thickness.

Correct AnswerverifiedShow Answer

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Correct AnswerverifiedB

Light rays are refracted (bent)when they cross the interface between materials with different refractive indices.

Correct AnswerverifiedShow Answer

In the Gram-staining procedure,the counterstain is

Correct AnswerverifiedShow Answer

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n) __________ microscope.

Correct AnswerverifiedShow Answer

An electron microscope uses __________ lenses to focus beams of electrons onto a specimen.

Correct AnswerverifiedShow Answer

The designer of the first transmission electron microscope,_________________,was awarded the 1986 Nobel Prize in physics.

Correct AnswerverifiedShow Answer

If the decolorizer is not left on long enough in the Gram-staining procedure,gram-positive organisms will be stained and gram-negative organisms will be stained .

Correct Answer
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After the secondary stain has been added,gram-positive organisms are stained and gram-negative organisms are stained .

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C

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A 45 $\times$ objective and a 10 $\times$ ocular produce a total magnification of

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True

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B

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