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A nucleotide is made up of a phosphate group, a five-carbon sugar molecule, and


A) two of five nitrogeneous bases.
B) one of five nitrogeneous bases.
C) one of four nitrogeneous bases.
D) two of four nitrogeneous bases.
E) three of six nitrogeneous bases.

F) B) and C)
G) C) and E)

Correct Answer

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C

Hydrogen bonds can form between guanine and ____, and between adenine and ____.


A) phosphate; sugar
B) thymine; cytosine
C) cytosine; thymine
D) sugar; phosphate
E) adenine; guanine

F) A) and B)
G) A) and E)

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C

Match the scientist(s) with their corresponding revolutionary discovery or experiment.

Premises
demonstrated that DNA is replicated semiconservatively
Responses
Griffith
Avery
Meselson and Stahl
Chargaff
Hershey and Chase

Correct Answer

Griffith
Avery
Meselson and Stahl
Chargaff
Hershey and Chase

Match the scientist(s) with their corresponding revolutionary discovery or experiment.

Premises
demonstrated the ratio of nucleotide bases in DNA
Responses
Chargaff
Avery
Meselson and Stahl
Griffith
Hershey and Chase

Correct Answer

Chargaff
Avery
Meselson and Stahl
Griffith
Hershey and Chase

In the experiments of Griffith, the conversion of nonlethal R-strain bacteria to lethal S-strain bacteria


A) was due to genetic mutation.
B) was due to transformation.
C) proved that proteins are the genetic material.
D) could not be reproduced by other researchers.
E) was similar to experiments performed by Watson and Crick.

F) D) and E)
G) A) and E)

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In replication, once the DNA strands have been separated, reformation of the double helix is prevented by


A) DNA helicase enzyme.
B) single-strand binding proteins.
C) DNA polymerases.
D) ATP.
E) DNA primase.

F) B) and C)
G) C) and D)

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X-ray diffraction images produced by ____ were used by Watson and Crick to infer the structure of DNA.


A) Wilkins
B) Griffith
C) Franklin
D) Hershey
E) Watson and Wilkins

F) B) and D)
G) A) and E)

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Primase is the enzyme responsible for


A) unwinding the DNA double strand to allow DNA polymerase access to the template DNA.
B) introducing nicks into the DNA double strand in order to prevent the formation of knots.
C) hydrolyzing ATP to facilitate DNA unwinding.
D) making short strands of RNA at the site of replication initiation.
E) forming a replication fork in the DNA double helix.

F) B) and D)
G) B) and C)

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Enzymes called ____ form breaks in the DNA molecules to prevent the formation of knots in the DNA helix during replication.


A) topoisomerases
B) single-strand binding proteins
C) DNA polymerases
D) RNA polymerases
E) DNA ligases

F) B) and D)
G) B) and E)

Correct Answer

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In the following questions, match the component of DNA replication with its function.

Premises
catalyzes the synthesis of an RNA primer at both leading and lagging strand
Responses
DNA primase
Single-strand binding protein
DNA polymerase
DNA helicase
Topoisomerase
DNA ligase

Correct Answer

DNA primase
Single-strand binding protein
DNA polymerase
DNA helicase
Topoisomerase
DNA ligase

Which is a powerful method for determining the 3D structure of a molecule?


A) NMR spectroscopy
B) 3D electron microscopy
C) Infrared spectroscopy
D) Ultraviolet spectroscopy
E) X-ray diffraction

F) None of the above
G) A) and B)

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Briefly explain the experiments of Avery and his colleagues and those of Hershey and Chase. What did those experiments support?

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Concepts to Consider: The experiments of...

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Match the scientist(s) with their corresponding revolutionary discovery or experiment.

Premises
demonstrated that genetic traits can be transferred from one bacterium to another
Responses
Meselson and Stahl
Griffith
Hershey and Chase
Avery
Chargaff

Correct Answer

Meselson and Stahl
Griffith
Hershey and Chase
Avery
Chargaff

From the DNA X-ray crystallography data, Franklin and Wilkins inferred that ____, while Watson and Crick determined that ____.


A) purines and pyrimidines exist in a 1:1 ratio; DNA is helical
B) phosphates are stacked liked rungs on a ladder; DNA is helical
C) DNA is helical; DNA is the genetic material
D) DNA is helical; the flat nucleotide bases are stacked upon each other
E) the flat nucleotide bases are stacked upon each other; DNA is helical.

F) A) and C)
G) A) and B)

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How is a bacterial chromosome replicated?


A) The linear DNA molecule is replicated from multiple origins of replication bidirectionally.
B) The linear DNA molecule is replicated from one origin of replication bidirectionally.
C) The circular DNA molecule is replicated from multiple origins of replication bidirectionally.
D) The circular DNA molecule is replicated from one origin of replication bidirectionally.
E) The circular DNA molecule is replicated from one origin of replication unidirectionally.

F) C) and D)
G) A) and E)

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Which of the following consists of short, noncoding, guanine-rich DNA sequences that repeat many times?


A) DNA ligase
B) Helicase
C) Telomere
D) Plasmid
E) Apoptosis

F) B) and D)
G) A) and D)

Correct Answer

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Match the scientist(s) with their corresponding revolutionary discovery or experiment.

Premises
discovered that the genetic material in bacteriophages is DNA
Responses
Hershey and Chase
Avery
Meselson and Stahl
Chargaff
Griffith

Correct Answer

Hershey and Chase
Avery
Meselson and Stahl
Chargaff
Griffith

The ends of eukaryotic chromosomes can be lengthened by


A) apoptosis.
B) mismatch repair enzymes.
C) primase.
D) telomerase.
E) DNA polymerase.

F) None of the above
G) A) and B)

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Which of the following nucleotide sequences represents the complement to the DNA strand, 5′-AGATCCG-3′?


A) 5′-AGATCCG-3′
B) 3′-AGATCCG-5′
C) 5′-CTCGAAT-3′
D) 3′-CTCGAAT-5′
E) 3′-TCTAGGC-5′

F) A) and E)
G) A) and B)

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E

Which of the following causes the unwinding of the DNA double helix?


A) DNA polymerase
B) DNA helicase
C) RNA primer
D) Primosome
E) RNA polymerase

F) B) and D)
G) C) and D)

Correct Answer

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