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Isozymes are different proteins from one species which catalyze different chemical reactions.

A) True
B) False

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Mutases are described as


A) polymerases that catalyze phosphoryl group transfers.
B) isomerases that catalyze the transfer of phosphoryl groups from one part of a substrate molecule to another.
C) forming intermediate free phosphate (Pi) .
D) All of the above.

E) None of the above
F) B) and D)

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Phosphofructokinase I deficiency results in


A) an overproduction of Fructose 6-phosphate.
B) an overproduction of Fructose 1,6-bisphosphate.
C) fructose 1,6-bisphosphate deficiency.
D) A and C only.
E) All of the above.

F) C) and D)
G) A) and B)

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2,3-bisphosphoglycerate is


A) an essential component of glycolysis for ATP production.
B) converted to 3-phosphoglycerate with formation of ATP.
C) essential for the efficient release of O² from hemoglobin.
D) a product of the enzyme phosphofructokinase.

E) None of the above
F) A) and D)

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Two molecules of ATP are consumed per glucose during the triose stage of glycolysis.

A) True
B) False

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PFK-2 and fructose 2,6-bisphosphatase are two names for the same enzyme.The name PFK-2 is used for the enzyme's catalysis of the phosphorylation of fructose 6-phosphate to fructose 2,6-bisphosphate.The name fructose 2,6-bisphosphatase is used for its catalysis of the reverse reaction.What is unique about this enzyme that makes it logical to use two names?


A) It is one of very few enzymes that can catalyze both the forward and reverse reactions.
B) The enzyme is a monomer when catalyzing the phosphorylation reaction and a dimer when catalyzing the reverse reaction.
C) The forward and reverse reactions occur in different compartments within the cell,so a different name is used for each activity.
D) The enzyme is bifunctional.The forward and reverse reactions are catalyzed by different sites on the same enzyme.

E) B) and D)
F) A) and D)

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Lactic acid produced by certain bacteria is used to curdle milk during cheese-making.

A) True
B) False

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An increase in blood insulin concentration increases the rate of glucose uptake in adipose tissue and striated muscle cells.

A) True
B) False

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Which must be satisfied to obtain net flux toward product in a metabolic pathway?


A) Each step in the pathway has an actual free energy change that is either negative or zero.
B) The overall actual free energy change is negative.
C) The net enthalpy is endothermic.
D) A and B above.
E) All of the above.

F) A) and C)
G) D) and E)

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The genetic disorder galactosemia can cause ________ in infants.


A) inability to properly digest milk due to its galactose content
B) jaundice
C) damage to the nervous system
D) liver damage
E) All of the above

F) A) and C)
G) C) and D)

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Which will be the main energy-producing degradation pathway for E.coli being grown on a medium where gluconate is the only carbon source?


A) Embden-Meyeroff-Parnas.
B) Entner-Doudoroff.
C) Pentose phosphate.
D) Gycolytic.

E) A) and D)
F) B) and D)

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How would the liver normally respond under condition of high glucose?


A) Phosphorylating glucose for entry into the glycolytic pathway.
B) Saturating glucokinase with glucose.
C) Phosphorylating glucose for entry into the glycogen synthesis pathways.
D) A and B only.
E) A and C only.

F) D) and E)
G) A) and E)

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Arsenate


A) competes with NAD+ for the binding site in 1,3-bisphosphoglycerate.
B) competes with phosphate for its binding site in glyceraldehyde 3-phosphate dehydrogenase.
C) produces a stable analog of 1,3-bisphosphoglycerate.
D) All of the above.

E) None of the above
F) A) and B)

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Seven of the ten reactions in the glycolytic pathway have free energy values close to zero.What does this tell us about those reactions?


A) They are near equilibrium reactions.
B) They are not control points for pathway regulation.
C) They are reversible reactions.
D) All of the above.
E) None of the above.

F) A) and E)
G) D) and E)

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Glucagon triggers the adenylate cyclase signally pathway.This activates protein kinase A which phosphorylates serine residues.

A) True
B) False

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Under what situation might lactic acidosis occur?


A) Lactate dehydrogenase is inactive.
B) Transport of glucose into cells is accelerated.
C) Oxygen supply to tissues is inadequate.
D) PFK-1 is over-activated.

E) A) and B)
F) A) and C)

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Insulin increases the capacity of a cell to transport glucose by


A) forming more GLUT 4 vesicles.
B) stimulating hexokinase.
C) stimulating GLUTs 1,3 and others of the GLUT family.
D) stimulating GLUT 4 vesicles to fuse with the plasma membrane.

E) None of the above
F) A) and B)

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The enzyme that catalyzes the conversion of pyruvate to lactate is ________.


A) lactate reductase
B) pyruvate kinase
C) lactoenolpyruvate
D) lactate dehydrogenase

E) A) and D)
F) A) and C)

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Isozymes are enzymes


A) from one species that catalyze the same reaction.
B) from different species that catalyze the same reaction.
C) that have isomers as substrates.
D) that have products that are isomers.

E) B) and D)
F) B) and C)

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Glucose 6-phosphate allosterically inhibits


A) hexokinase I.
B) glucokinase.
C) hexokinase II.
D) All of the above
E) A and C only

F) B) and C)
G) B) and E)

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